User Guide Kit 103591-100 – Agilent, ATP rate assay kit, Seahorse Real-Time ATP rate assay kit …
User Guide Kit 103591-100 – Agilent, Seahorse XF ATP | Agilent, Seahorse XF Real-Time ATP Rate Assay Kit. The Agilent Seahorse ATP Real-Time rate assay is the only assay that quantifies the rate of adenosine triphosphate (ATP) production from glycolysis and mitochondria simultaneously using label-free technology in live cells. The real-time kinetic quantification delivers a dynamic picture of cellular …
The Agilent Seahorse XF Real-Time ATP rate assay allows you to measure what matters. Now you can measure the rate of ATP production from the two key energy pathways (glycolysis and mitochondrial respiration) simultaneously in live cells using label-free technology.
The Seahorse XFp Real-Time ATP Rate Assay uses an average P/O value of 2.75 for these calculations that was validated to accurately represents the assay conditions for a broad panel of cells under different fuels availabilities (see White Paper: Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology ).
Accelerate your research using the Seahorse XF Real Time ATP Rate Assay Report Generator. XF Real-Time ATP Rate Assay Report Generator | Agilent Skip to main content Skip to main content, RUO. The Agilent Seahorse XF Real-Time ATP rate assay report generator is the data analysis software for the Agilent Seahor se XF Real-Time ATP rate assay. This report generator automatically converts experimentally derived oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) data into total ATP production rates, and …
ATP production to maintain total intracellular ATP levels. The Agilent Seahorse XF Real-Time ATP Rate Assay is designed to measure total ATP production rates in living cells. Even more relevant is the ability of this assay to distinguish between the fractions of ATP that are produced from, The Agilent Seahorse XF ATP Real-Time rate assay is the only assay that measures and quantifies the rate of ATP ( adenosine triphosphate ) production from glycolysis and mitochondria simultaneously using label-free technology in live cells, in real time . This assays can be used as a starting point to understand metabolism-supported cellular function.
Agilent Seahorse XF Real-Time ATP Rate Assay Kit FAQs How is the mitoATP Production Rate calculated if there are different oxidizable substrates (with different P/O ratios) in the assay media? To convert ATP -coupled respiration (OCR) into mitochondrial ATP production rates, the stoichiometry of ADP phosphorylated to ATP per atom of oxygen reduced at the terminal point of electronic transport.
The XF Real-Time ATP Rate Assay Report Generator uses PER data exported from Wave 2.6 to calculate glycoATP Production Rates. If Buffer Factor is not assigned to assay media in Wave 2.6 before export, then the Report Generator will not calculate glycoATP Production Rates.
Seahorse XF Real-Time ATP Rate Assay Kit. Each kit contains six (6) single-use pouches. Each pouch contains one (1) each of oligomycin and rotenone/antimycin A. For use with Seahorse XFe/XF96 and XFe24 analyzers. Add to Favorites. + Create New list.
